Edman degradation and protein sequencing: Pfu Methionine Aminopeptidase
Pfu Methionine Aminopeptidase is a thermostable methionine aminopeptidase isolated from Pyrococcus furiosus and expressed as a recombinant protein. It specifically liberates the N-terminal methionine residue from proteins and peptides during Edman degradation, enabling protein sequencing.
- Release of N-terminal methionine residues from recombinant proteins and peptides
Escherichia coli transformed with Pyrococcus furiosus methionine aminopeptidase gene.
≥95% via SDS-PAGE. No other proteases detected.
- Molecular weight: 32.848 (MS analysis), 37.000 (SDS-PAGE)
- Optimum temperature: 85–95°C
- Thermostability: Stable for 1 hr at 75°C, pH 7.2 and 0.5 mM Co2+
- Optimum pH: 7.0–8.0
- Tolerance to denaturants: ≤2 M Urea, ≤0.2 M Guanidine-HCl, ≤0.01% SDS
- Activator: Co2+
- Inhibitor: EDTA
Solution of 10 mM Tris-HCl, pH 7.5, containing 0.01% Tween 20 and 0.1 mM CoCl2
Ben-Bassat, A. et al. Processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structure. J. Bacteriol. 169, 751–757 (1987).
Ben-Bassat, A. et al. Amino-terminal processing of proteins. Nature 326, 315 (1987).
Miller, C.G. et al. N-terminal methionine-specific peptidase in Salmonella typhimurium. Proc. Natl. Acad. Sci. USA 84, 2718–2722 (1987).
Yasueda, H. et al. High-level direct expression of semi-synthetic human interleukin-6 in Escherichia coli and production of N-terminus met-free product. Biotechnology 8, 1036–1040 (1990).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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