Cryonase Cold-Active Nuclease
Cryonase Cold-Active Nuclease is a recombinant endonuclease originally derived from a psychrophile, Shewanella sp., and purified from E. coli. This endonuclease enzyme can complete digestion of all types of DNA and RNA (single-stranded, double-stranded, linear or circularized) at cold temperatures and even in samples placed on ice. As a result, Cryonase Cold-Active Nuclease is frequently used during DNA digestion of mixed samples, such as those that include heat-labile proteins.
- Digestion of all types of DNA
- Digestion of all types of RNA
- Enzymatic and other heat-labile reactions
Purified from E. coli
- Protease activity is less than the detection limit
- E. coli genomic DNA contamination is less than the detection limit
Supplied in a buffer (20 mM NaCl, 10 mM Tris-HCl (pH 7.5), 50% glycerol, 2mM MgCl2)
One unit is defined as the amount of enzyme required to increase 260 nm absorbance by 0.001 per minute at 37°C and pH 7.5 using salmon sperm DNA as the substrate.
Baldermann, S. et al. Functional characterization of a carotenoid cleavage dioxygenase 1 and its relation to the carotenoid accumulation and volatile emission during the floral development of Osmanthus fragrans Lour. J. Exp. Bot. 61(11):2967–2977 (2010).
Yang, H. et al. Full Genome Sequence of Bluetongue Virus Serotype 4 from China. J. Virol. 86(23):13122–13123 (2012).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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