UGT1A9 plays a role in the regulation of cellular homeostasis by limiting drug-induced stress. Our workflow for adding a myc tag to the UGT1A9 gene began with hiPS cells cultured in our Cellartis DEF-CS 500 Culture System, which provided a homogeneous, undifferentiated starting population. We delivered the Cas9-sgRNA complex in the form of ribonucleoprotein (RNP) in order to decrease off-target effects and for footprint-free genome editing. Together with the RNP complex, we electroporated a 200-nucleotide long ssDNA encoding the myc tag. After editing, cells from the now heterogeneous population (consisting of wildtype, knockout, and successfully tagged cells) were single-cell isolated in a 96-well plate and expanded to generate clonal cell lines. The clonal cell lines were then screened to identify clones with the correct insertion.