Our workflow for the introduction of a tyrosinemia-related SNP c.786G>A (p.Trp262Ter) into the FAH (fumarylacetoacetate hydrolase) gene began with hiPS cells cultured in our Cellartis DEF-CS 500 Culture System, which provided a homogeneous, undifferentiated starting population. We used electroporation to deliver Cas9-sgRNA together with the HDR template, an ssDNA donor template of 200 nucleotides in length encoding the SNP of interest. We delivered the Cas9-sgRNA complex in the form of ribonucleoprotein (RNP) in order to decrease off-target effects and for footprint-free genome editing. Following electroporation, we screened the population of edited hiPS cells using our own SNP detection system. Single cells were seeded and expanded to generate clonal cell lines, and the lines were screened to identify clones with the desired c.786G>A substitution. No preselection was required prior to screening. We then used our efficient hepatocyte differentiation protocol to generate functional hepatocytes.