We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. Accept
  •  Customer Login
  • Register
  •  View Cart (0)
  •  Customer Login
  • Register
  •  View Cart (0)

  • Products
  • Learning centers
  • Services & Support
  • Areas of interest
  • About

Close

  • ‹ Back to Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
Blog post about Marson paper Blog post: CRISPR takes a giant step towards the clinic
Gene function learning center Gene function learning center

Contact us

Home › Areas of interest › Cancer research › Gene editing for cancer therapy/drug discovery

Cancer research

  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
Need help?
Contact Sales
Blog post about Marson paper Blog post: CRISPR takes a giant step towards the clinic
Gene function learning center Gene function learning center

Contact us

Gene editing for cancer therapy/drug discovery

CRISPR/Cas9 gene editing has become a powerful method to edit the genomes of many different organisms. First discovered in bacteria as part of an adaptive immune system, CRISPR/Cas9 and modified versions thereof are now broadly used to engineer genomes and to activate or repress the expression of specific genes. Furthermore, CRISPR/Cas9 gene editing promises to accelerate cancer research by providing an efficient technology to dissect mechanisms of tumorigenesis, identify targets for drug development, and possibly arm cells for cell-based therapies (Moses et al. 2018).

Highlighted products

Cancer therapy 

Genome editing approaches have enormous potential for targeted, locus-specific cancer treatments. The human papillomavirus (HPV) genes E6 and E7 contribute to the hallmark of resisting cell death by disrupting regular cell cycle and tumor suppressor function. Cas9-mediated HPV E7 oncogene disruption leads to significant inhibition of HPV-induced cancerous activity both in vitro and in vivo, as described by Lao et al., 2018. The authors used our Guide-it Mutation Detection Kit and Guide-it Indel Identification Kit to check for efficient gene editing.

Gene editing in cancer immunotherapy

Genome editing approaches have also shown promising results in cancer immunotherapy, to oppose the cancer hallmark of evading immune destruction. Modified chimeric antigen receptor (CAR) T cells have been generated for improved cancer targeting and destruction. Knockin genome modifications in T cells have also been generated with Cas9-sgRNA ribonucleoprotein (RNP) complexes. Kagoya et al., 2018, report that inhibiting DOT1L, a histone H3-lysine 79 methyltransferase, alleviates allogeneic T-cell responses. The authors used the Guide-it sgRNA In Vitro Transcription Kit and Guide-it Recombinant Cas9 (Electroporation-Ready) for CRISPR-mediated TCR ablation in CAR-T cells. Using electroporation of RNP complexes, they could achieve ~30% TCR knockout efficiency in CAR-T cells.

In a groundbreaking study recently published in the journal Nature, Prof. Alexander Marson and colleagues describe a highly efficient method for T-cell engineering that circumvents viral delivery and minimizes cellular toxicity by employing electroporation of Cas9-sgRNA RNP complexes in tandem with double-stranded DNA or single-stranded DNA (ssDNA) HDR templates (Roth et al. 2018).

Drug discovery

Genome-wide knockout screens are a powerful functional genomics tool to discover novel drug targets for cancer therapy. For pooled knockout screens with CRISPR/Cas9, a cell population with a diversity of gene knockouts needs to be generated. Lentiviral particles encoding an sgRNA library are used to infect Cas9-expressing cells at a low multiplicity of infection so that every cell potentially carries a distinct sgRNA cassette and specific gene knockout. Subsequently, this pool of knockout cells is exposed to selected perturbations, followed by NGS analysis compared to a reference control cell population. By this means, it is possible to monitor the phenotypic effect of specific gene knockouts within the cell population.

The Guide-it CRISPR Genome-Wide sgRNA Library System is a pooled lentiviral sgRNA library targeting the whole human genome for knockout screens and thus serves as an ideal tool to discover novel drug targets for cancer therapy (Figure 1). The library contains sgRNAs from the Brunello library, based on a recent algorithm for optimized guide sequences for each gene (Doench et al. 2016, Doench et al. 2018):
• four guides per gene
• 76,610 guides in total (includes 172 negative controls)
• 19,114 genes targeted

Pooled sgRNA library screen

Figure 1. Schematic of a pooled sgRNA library screen for 6-thioguanine resistance.


References and product citations

Doench J. G. et al. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat. Biotechnol. 34, 184–191 (2016).

Doench J. G. et al. Am I ready for CRISPR? A user's guide to genetic screens. Nat. Rev. Genet. 19, 67–80 (2018).

Kagoya Y. et al. DOT1L inhibition attenuates graft-versus-host disease by allogeneic T cells in adoptive immunotherapy models. Nat. Communications 9, 1915 (2018).

Lao Y. H. et al. HPV Oncogene Manipulation Using Nonvirally Delivered CRISPR/Cas9 or Natronobacterium gregoryi Argonaute. Adv. Sci. 1700540 (2018).

Moses C. et al. Hallmarks of cancer: The CRISPR generation. Eur. J. Cancer 93, 10–18 (2018).

Roth, T. L. et al. Reprogramming human T cell function and specificity with non-viral genome targeting. Nat. Lett. 559, 405–409 (2018).


Featured products

Cat. # Product Size Price License Quantity Details
632646 Guide-it™ CRISPR Genome-Wide sgRNA Library System 5 Screens USD $3876.00

License Statement

ID Number  
89 This product is protected by U.S. Patent 9,945,850 and additional pending patent. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
47 Portions of this product are covered by several patent applications owned by, or licensed to, GE Healthcare Dharmacon Inc. The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) under these patent rights to perform the viral infection methods using the lentiviral vectors claimed in those patent applications for research purposes solely in conjunction with this product. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include nor carry any right or license to use, develop, or otherwise exploit this product commercially, and no other rights are conveyed to the buyer to use the product or components of the product for any other purposes, including without limitation, provision of services to a third party, generation of commercial databases, or clinical diagnostics or therapeutics. This product is sold pursuant to a license from GE Healthcare Dharmacon Inc, and GE Healthcare Dharmacon Inc., reserves all other rights under these patent rights. For information on purchasing a license to the patent rights for uses other than in conjunction with this product or to use this product for purposes other than research, please contact GE Healthcare Dharmacon Inc., LSlicensing @ge.com.
63 Use of this product is covered by one or more of the following U.S. Patent Nos. and corresponding patent claims outside the U.S.: 6,998,115; 7,427,394. This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic use, (iii) screening of chemical and/or biological compounds for the identification of pharmaceutically active agents (including but not limited to screening of small molecules), target validation, preclinical testing services, or drug development. Any use of this product for any of the above mentioned purposes requires a license from the Massachusetts Institute of Technology.
272 This product (“Product”) and its use, is the subject of U.S. Patents 8,697,359 and 8,771,945 and pending U.S. Patent applications. The purchase of the Product conveys to the buyer the non-transferable right to use Product(s) purchased from Takara Bio USA, Inc. or its Affiliates, and any progeny, modification or derivative of a Product, or any cell or animal made or modified through use of a Product, or any progeny, modification or derivative of such cell or animal (“Related Material”), solely for research conducted by the buyer in accordance with all of the following requirements. No right is given to use this Product or Related Material for any other purpose, including, but not limited to, use in drugs, in vitro diagnostic purposes, therapeutics, or in humans. The buyer shall not sell or otherwise transfer Products (including without limitation any material that contains a Licensed Product in whole or part) or any Related Material to any other person or entity, or use Products or any Related Material to perform services for the benefit of any other person or entity, (ii) the buyer shall use only the purchased amount of the Products and components of the Products, and shall use any Related Material, only for its internal research and not for (a) the practice, performance or provision of any method, process or service, or (b) the manufacture, sale, use, distribution, disposition or importing of any product, in each case (a) or (b) for consideration, or on any other commercial basis (“Commercial Purpose”), (iii) the buyer shall use Licensed Products and any Related Material in compliance with all applicable laws and regulations, including without limitation applicable human health and animal welfare laws and regulations, and (iv) the buyer shall indemnify, defend and hold harmless MIT, Harvard and The Broad and their current and former trustees, directors, officers, faculty, affiliated investigators, students, employees, and agents and their respective successors, heirs and assigns (“Indemnitees”), against any liability, damage, loss, or expense (including without limitation reasonable attorneys’ fees and expenses) incurred by or imposed upon any of the Indemnitees in connection with any claims, suits, investigations, actions, demands or judgments arising out of or related to the exercise of any rights granted to the buyer, or any breach of the rights granted hereunder by the buyer.
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,005,511; 7,157,566; 7,393,923 and 7,250,298.
330 This product is the subject of a technology license agreement for internal research use only. Use of this product other than for research use may require additional licenses. Information on license restrictions or for uses other than research may be obtained by contacting licensing@takarabio.com.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the Guide-it™ CRISPR Genome-Wide sgRNA Library System product License Agreement can be found by clicking here.
55 cPPT Element. This product and its use are the subject to one or more of the following U.S. Pat. 8,093,042 and foreign equivalents. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Takara Bio USA, Inc. is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).
*

The Guide-it CRISPR Genome-Wide sgRNA Library System includes all of the components necessary to perform five lentiviral-based, genome-wide CRISPR/Cas9 knockout screens in human cells. Provided with the kit are lentiviral vectors for expression of Cas9 and >76,000 sgRNAs targeting >19,000 human genes (four guides per gene) in lyophilized, ready-to-transfect formats that simplify the production of high-titer lentivirus. The sgRNA library included with this kit is based on the Brunello library (Doench et al. 2016), and incorporates design features intended to maximize on-target efficacy and minimize off-target effects. To ensure balanced guide representation, we analyze the sgRNA library by NGS to confirm that >90% of the included sgRNAs fall within a 10-fold distribution range. Enrichment or depletion of sgRNAs in screened cells can be analyzed by NGS with the Guide-it CRISPR Genome-Wide sgRNA Library NGS Analysis Kit (Cat. # 632647).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

Representation of sgRNAs within the Guide-it CRISPR Genome-Wide sgRNA Library System

Representation of sgRNAs within the Guide-it CRISPR Genome-Wide sgRNA Library System

Representation of sgRNAs within the Guide-it CRISPR Genome-Wide sgRNA Library System. Panel A. Guide representation in plasmid library. The Brunello-based sgRNA library was cloned into the pLVXS-sgRNA-mCherry-hyg Vector and amplified. The representation of the sgRNAs within the plasmid DNA library was verified by NGS. The representation of >90% of all the stated sgRNAs within the library was within a 10-fold distribution range. Bars represent the number of sgRNAs with a specific read count. Panel B. Comparison of guide representation in the plasmid library and transduced cells. Genomic DNA was isolated from Cas9+/sgRNA+ A375 cells selected on hygromycin and PCR amplified. The PCR product was sequenced to determine read counts of each integrated sgRNA relative to the starting plasmid DNA population. We observed strong Spearman and Pearson correlations indicating that the system is able to maintain sgRNA representation in the transduced and selected cell population.

Back

Identification and analysis of sgRNAs isolated from cells after a 6-thioguanine screen

Identification and analysis of sgRNAs isolated from cells after a 6-thioguanine screen

Identification and analysis of sgRNAs isolated from cells after a 6-thioguanine (6-TG) screen. Panel A. sgRNA representation was compared between the original plasmid library population used to make the lentivirus preparation and the resulting transduced, selected and non-selected cell populations. All four sgRNAs for HPRT (red dots) and NUDT5 (black dots) were enriched in the 6-TG-selected population (blue dots). The representation of these sgRNAs in the plasmid library (gray) and transduced, non-selected population (orange) is also shown. Panel B. After selection, the sgRNA representation was shifted in response (blue) to 6-TG. Individual sgRNAs that were enriched are highlighted within this correlation (red and orange dots). Inset shows fold-enrichment of the four sgRNAs for the HPRT gene as compared to the non-selected population.

Back

Workflow schematic for an sgRNA library screen using 6-thioguanine (6-TG) selection

Workflow schematic for an sgRNA library screen using 6-thioguanine (6-TG) selection
Workflow schematic for an sgRNA library screen using 6-thioguanine (6-TG) selection. After transduction of Cas9+ A375 cells with the sgRNA library, hygromycin selection, and expansion, the Cas9+/ sgRNA+ cells were split into two populations. A part of the Cas9+/sgRNA+ cell population was exposed to 6-TG, while the control population was expanded under normal culture conditions, without adding 6-TG. After expansion of both cell populations, genomic DNA was harvested and prepared for sequencing with the Guide-it CRISPR Genome-Wide sgRNA Library NGS Analysis Kit.

Back

Determination of transduction efficiency of the sgRNA library lentivirus using mCherry fluorescence

Determination of transduction efficiency of the sgRNA library lentivirus using mCherry fluorescence

Determination of transduction efficiency of the sgRNA library lentivirus using mCherry fluorescence. Lentivirus containing the sgRNA library was produced following the instructions in the user manual. Lentivirus was harvested after 48 hrs and used to transduce Cas9-expressing A375 cells at varying MOIs. Transduced cells were plated and analyzed for transduction efficiency by fluorescence microscopy and FACS after 48 hours.

Back

Vector and sgRNA scaffold design used in the Guide-it CRISPR sgRNA library

Vector and sgRNA scaffold design used in the Guide-it CRISPR sgRNA library

Vector and sgRNA scaffold design used in the Guide-it CRISPR sgRNA library. Panel A. The pLVXS-EF1a-Cas9-PGK-Puro and pLVXS-sgRNA-mCherry-hyg vectors maps showing the lentiviral vector backbone. The vectors are self-inactivating for increased safety during production and use. The pLVXS-sgRNA-mCherry-hyg vector contains both mCherry and hygromycin markers expressed from an IRES-linked bicistronic expression cassette. The sgRNAs are expressed from a human U6 promoter and contain an optimized scaffold sequence for better Cas9 loading and editing efficiency (Panel B).

631444 Guide-it™ Indel Identification Kit 10 Rxns USD $399.00

License Statement

ID Number  
28 This product is covered by the claims of U.S. Patent No. 7,575,860 and its foreign counterpart patent claims.

The Guide-it Indel Identification Kit is used for characterization of insertions and deletions (indels) generated by gene editing tools, such as CRISPR/Cas9. This kit contains all of the components needed to amplify, clone, and prepare modified target sites for DNA sequence analysis. This kit uses Terra PCR Direct to amplify targets directly from crude genomic DNA extracts. The resulting pool of fragments, which may contain a variety of indels, are cloned into a prelinearized pUC19 vector using the In-Fusion cloning system. Colony PCR of individual clones using Terra PCR Direct followed by DNA sequencing allows indel characterization.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

Back

Identification of insertions and deletions (indels) in the CD81 gene after CRISPR/Cas9 targeting

Identification of insertions and deletions (indels) in the CD81 gene after CRISPR/Cas9 targeting
Identification of insertions and deletions (indels) in the CD81 gene after CRISPR/Cas9 targeting. HeLa cells were transfected with plasmids encoding Cas9 and an sgRNA targeting the CD81 gene. The Guide-it Indel Identification Kit was used to prepare CD81 target sites for DNA sequence analysis. The sequencing data from six clones was aligned with the wild-type sequence, revealing a broad range of indels in the CD81 gene.

Back

The Guide-it Indel Identification Kit provides a complete workflow for identifying the variety of insertions and deletions (indels) introduced by nuclease-based genome editing

The Guide-it Indel Identification Kit provides a complete workflow for identifying the variety of insertions and deletions (indels) introduced by nuclease-based genome editing

The Guide-it Indel Identification Kit provides a complete workflow for identifying the variety of insertions and deletions (indels) introduced by nuclease-based genome editing. The protocol uses direct PCR to amplify a genomic DNA fragment (~500 to 700 bp) containing the target site directly from crude cell lysates (step 1). The resulting amplified fragments contain a pool of edited target sites from individual cells. These PCR products are cloned directly into a pre-linearized pUC19 vector using the In-Fusion Cloning system (step 2). After transformation of an optimized E. coli strain, colony PCR is used to amplify the target site from the plasmid (step 3). DNA sequencing is then used to identify the different indels generated at the targeted genomic site (step 4)

Back

The CRISPR/Cas9 system, a simple, RNA-programmable method to mediate genome editing in mammalian cells

The CRISPR/Cas9 system, a simple, RNA-programmable method to mediate genome editing in mammalian cells

The CRISPR/Cas9 system, a simple, RNA-programmable method to mediate genome editing in mammalian cells. The CRISPR/Cas9 system relies on a single guide RNA (sgRNA) directing the Cas9 endonuclease to induce a double strand break at a specific target sequence three base-pairs upstream of a PAM sequence in genomic DNA. This DNA cleavage can be repaired in one of two ways: 1) nonhomologous end joining, (NHEJ) resulting in gene knockout due to error-prone repair (orange), or 2) homology-directed repair (HDR), resulting in gene knockin due to the presence of a homologous repair template (purple).

632644 Guide-it™ Long ssDNA Production System 25 Rxns USD $469.00

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 
272 This product (“Product”) and its use, is the subject of U.S. Patents 8,697,359 and 8,771,945 and pending U.S. Patent applications. The purchase of the Product conveys to the buyer the non-transferable right to use Product(s) purchased from Takara Bio USA, Inc. or its Affiliates, and any progeny, modification or derivative of a Product, or any cell or animal made or modified through use of a Product, or any progeny, modification or derivative of such cell or animal (“Related Material”), solely for research conducted by the buyer in accordance with all of the following requirements. No right is given to use this Product or Related Material for any other purpose, including, but not limited to, use in drugs, in vitro diagnostic purposes, therapeutics, or in humans. The buyer shall not sell or otherwise transfer Products (including without limitation any material that contains a Licensed Product in whole or part) or any Related Material to any other person or entity, or use Products or any Related Material to perform services for the benefit of any other person or entity, (ii) the buyer shall use only the purchased amount of the Products and components of the Products, and shall use any Related Material, only for its internal research and not for (a) the practice, performance or provision of any method, process or service, or (b) the manufacture, sale, use, distribution, disposition or importing of any product, in each case (a) or (b) for consideration, or on any other commercial basis (“Commercial Purpose”), (iii) the buyer shall use Licensed Products and any Related Material in compliance with all applicable laws and regulations, including without limitation applicable human health and animal welfare laws and regulations, and (iv) the buyer shall indemnify, defend and hold harmless MIT, Harvard and The Broad and their current and former trustees, directors, officers, faculty, affiliated investigators, students, employees, and agents and their respective successors, heirs and assigns (“Indemnitees”), against any liability, damage, loss, or expense (including without limitation reasonable attorneys’ fees and expenses) incurred by or imposed upon any of the Indemnitees in connection with any claims, suits, investigations, actions, demands or judgments arising out of or related to the exercise of any rights granted to the buyer, or any breach of the rights granted hereunder by the buyer.
325 Patent pending. For further information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The Guide-it Long ssDNA Production System enables production of single-stranded DNA (ssDNA) oligos up to 5 kb in length for use as repair templates in knockin experiments involving CRISPR/Cas9 or other genome editing tools. Included with the system are sufficient reagents for production of 50 different ssDNA oligos (25 pairs of sense and antisense ssDNAs), and the NucleoSpin Gel and PCR Clean-up kit for purification of ssDNA prior to target-cell delivery.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

FACS analysis of AcGFP1 knockin efficiencies at the SEC61B and TUBA1A loci

FACS analysis of AcGFP1 knockin efficiencies at the SEC61B and TUBA1A loci

FACS analysis of AcGFP1 knockin efficiencies at the SEC61B and TUBA1A loci. Cas9-sgRNA RNPs targeting SEC61B and TUBA1A and corresponding AcGFP1 ssDNA homology-directed repair (HDR) templates generated using the Guide-it Long ssDNA Production System were electroporated into ChiPSC18 cells. Following culturing, electroporated target cells and non-electroporated negative control cells were analyzed for GFP expression by FACS. Using this approach, knockin efficiencies of 12.55% and 2.75% were observed for insertion of AcGFP1 at SEC61B and TUBA1A, respectively.

Back

PCR analysis of AcGFP1 knockin at the SEC61B locus in clonal populations isolated by FACS

PCR analysis of AcGFP1 knockin at the SEC61B locus in clonal populations isolated by FACS

PCR analysis of AcGFP1 knockin at the SEC61B locus in clonal populations isolated by FACS. ChiPSC18 cell populations were electroporated with Cas9-sgRNA RNPs and sense or antisense ssDNA HDR templates (labeled ssDNA-1 or ssDNA-2, respectively) that were designed for knockin of AcGFP1 at the SEC61B locus. The cells were sorted on the basis of GFP expression via FACS. Individual clones from the GFP-positive populations were isolated by limiting dilution and cultured to yield clonal cell populations. Genomic DNA extracted from the clonal populations was analyzed by PCR using primers (L-F and R-R, respectively) designed to anneal on either side of the GFP insertion site as shown in the schematic. The GFP and homology arm sequences included in the ssDNA templates are shown in green and light blue, respectively, while the genomic sequences outside the region targeted by the ssDNA homology arms are shown in dark blue. The PCR primers were designed so that the presence of the inserted AcGFP1 sequence yielded a 1.885-kb PCR product, while absence of the AcGFP1 insert yielded a 1.159-kb PCR product. Gel electrophoresis of PCR products confirmed the occurrence of monoallelic knockins of AcGFP1 at SEC61B, represented by double bands (ssDNA-1 Lane C5, and ssDNA-2 Lane C15), and biallelic knockins of AcGFP1 at SEC61B, represented by single bands (ssDNA-1 Lanes C1, C7, C8, C11, and ssDNA-2 Lanes C2, C6, C8, and C10). Two clones lacking any copies of the AcGFP1 insert were also identified (ssDNA-1 Lane C10, and ssDNA-2 Lane C11).

Back

Schematic depicting Cas9-sgRNA cleavage site and ssDNA construct designed for knockin of AcGFP1 at the SEC61B locus

Schematic depicting Cas9-sgRNA cleavage site and ssDNA construct designed for knockin of AcGFP1 at the SEC61B locus

Schematic depicting Cas9-sgRNA cleavage site and ssDNA construct designed for knockin of AcGFP1 at the SEC61B locus. The ssDNA construct generated using the Guide-it Long ssDNA Production System included the AcGFP1 coding sequence (~700 nt in length) flanked on either side by homology arms ~350 nt in length. The homology arms were designed to insert the AcGFP1 sequence immediately 5' relative to Exon 1 of SEC61B. As indicated, the ssDNA construct did not include a promoter sequence, so that expression of the resulting AcGFP1-SEC61B fusion protein would be driven by the endogenous SEC61B promoter. Binding sites for PCR primers used to detect the inserted AcGFP1 sequence are shown by blue arrows.

Back

Schematic of Cas9-sgRNA cleavage site and ssDNA construct designed for knockin of AcGFP1 at the TUBA1A locus

Schematic of Cas9-sgRNA cleavage site and ssDNA construct designed for knockin of AcGFP1 at the TUBA1A locus

Schematic of Cas9-sgRNA cleavage site and ssDNA construct designed for knockin of AcGFP1 at the TUBA1A locus. The ssDNA construct generated using the Guide-it Long ssDNA Production System included the AcGFP1 coding sequence (~700 nt in length) flanked on either side by homology arms ~350 nt in length. The homology arms were designed to insert the AcGFP1 sequence at the 5' end of Exon 2 of TUBA1A. As indicated, the ssDNA construct did not include a promoter sequence, so that expression of the resulting AcGFP1-TUBA1A fusion protein would be driven by the endogenous TUBA1A promoter.

Back

Toxicity comparison for electroporation of dsDNA or ssDNA

Toxicity comparison for electroporation of dsDNA or ssDNA

Toxicity comparison for electroporation of dsDNA or ssDNA. ChiPSC18 cells were electroporated with Cas9-sgRNA RNPs targeting the TUBA1A locus, in the absence or presence of homology-directed repair (HDR) templates consisting of dsDNA or ssDNA. After 48 hours of culturing, imaging was performed to compare the toxicity associated with each HDR template. While electroporation of either DNA template resulted in increased toxicity compared to electroporation of Cas9-sgRNA RNPs alone (as evidenced by lower cell counts following culturing), application of the ssDNA template resulted in greatly reduced toxicity compared to the dsDNA template.

Back

In vivo expression of AcGFP1 fusion proteins in cells edited using Cas9-sgRNA RNPs and ssDNA HDR templates

In vivo expression of AcGFP1 fusion proteins in cells edited using Cas9-sgRNA RNPs and ssDNA HDR templates

In vivo expression of AcGFP1 fusion proteins in cells edited using Cas9-sgRNA RNPs and ssDNA HDR templates. ChiPSC18 cells were edited using Cas9-sgRNAs and ssDNA HDR templates designed for knockin of AcGFP1 at the SEC61B and TUBA1A loci, respectively. GFP-positive clonal cell lines obtained from each edited population were then stained with DAPI and analyzed by fluorescence microscopy. Expression of GFP-SEC61B was observed in endosomal compartments (left), while GFP-TUBA1A localized to microtubules (right).

631448 Guide-it™ Mutation Detection Kit 25 Rxns USD $203.00

The Guide-it Mutation Detection Kit contains all the reagents needed for PCR-based identification of insertions or deletions generated during cellular non-homologous end joining (NHEJ) repair. The first step is the amplification of the putative target sequence directly from cells. This kit uses Terra PCR Direct Polymerase Mix and Buffer, so there is no need to extract genomic DNA from your cell population prior to amplification of your target sequence. The amplicon is then melted and hybridized to form the mismatched targets for cleavage by the Guide-it Resolvase. Sufficient material is provided for 100 amplification and cleavage reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The Guide-it Mutation Detection Kit is used to confirm the presence of mutations in genomic DNA

The Guide-it Mutation Detection Kit is used to confirm the presence of mutations in genomic DNA
The Guide-it Mutation Detection Kit is used to confirm the presence of mutations in genomic DNA. In the first step your target sequence is amplified directly from your target cells using the Terra PCR Direct Polymerase included in the kit, so there is no need to extract and purify genomic DNA from your cell population prior to amplification of your target sequence. The amplicon is then melted and hybridized to form the mismatched targets that can be cleaved by the Guide-it Resolvase.

Back

Comparison of the Guide-it and Surveyor assays for detecting mutations in mammalian cells

Comparison of the Guide-it and Surveyor assays for detecting mutations in mammalian cells

Comparison of the Guide-it and Surveyor assays for detecting mutations in mammalian cells. 293T cells were transfected with plasmids encoding Cas9 and a sgRNA specific for the AAVS1 locus. Transfected cells were harvested 48 hr post-transfection and mixed with untransfected cells at varying ratios. An amplicon containing the targeted AAVS1 locus was generated using Terra Direct Polymerase Mix, and the PCR products were purified and cleaved using either Guide-it Resolvase or the Cel1 enzyme (Surveyor assay). Mutations were easily discernable when using the Guide-it kit. In contrast, the Surveyor Assay showed considerable smearing, making it difficult to determine cleavage efficiency and reducing the ability to detect low levels of mutation.

Back

Successful knockout of AcGFP1 in HT1080 cells using the CRISPR/Cas9 system

Successful knockout of AcGFP1 in HT1080 cells using the CRISPR/Cas9 system

Successful knockout of AcGFP1 in HT1080 cells using the CRISPR/Cas9 system. Panel A. Schematic of the AcGFP DNA sequence and the location of sgRNAs tested and primer placement for the mutation detection assay. HT1080 cells containing a single copy of AcGFP1 were transfected with 1.5 μg of plasmid DNA for Cas9 expression and 1.5 μg of a plasmid harboring one of two sgRNAs (T1 or T2) using Xfect Transfection Reagent. The cell population was assayed 6 days post-transfection for cleavage efficiency and loss of fluorescence. Panel B. Using the Guide-it Mutation Detection Kit, cleavage products were detected for both sgRNAs, indicating that both CRISPRs successfully disrupted the AcGFP1 locus. Panel C. The AcGFP1 disruptions were functionally relevant, as a subpopulation of non-fluorescent cells could be detected by FACS.

Back

631448: Guide-it Mutation Detection Kit

631448: Guide-it Mutation Detection Kit
632641 Guide-it™ Recombinant Cas9 (Electroporation-Ready) 100 ug USD $205.00

License Statement

ID Number  
272 This product (“Product”) and its use, is the subject of U.S. Patents 8,697,359 and 8,771,945 and pending U.S. Patent applications. The purchase of the Product conveys to the buyer the non-transferable right to use Product(s) purchased from Takara Bio USA, Inc. or its Affiliates, and any progeny, modification or derivative of a Product, or any cell or animal made or modified through use of a Product, or any progeny, modification or derivative of such cell or animal (“Related Material”), solely for research conducted by the buyer in accordance with all of the following requirements. No right is given to use this Product or Related Material for any other purpose, including, but not limited to, use in drugs, in vitro diagnostic purposes, therapeutics, or in humans. The buyer shall not sell or otherwise transfer Products (including without limitation any material that contains a Licensed Product in whole or part) or any Related Material to any other person or entity, or use Products or any Related Material to perform services for the benefit of any other person or entity, (ii) the buyer shall use only the purchased amount of the Products and components of the Products, and shall use any Related Material, only for its internal research and not for (a) the practice, performance or provision of any method, process or service, or (b) the manufacture, sale, use, distribution, disposition or importing of any product, in each case (a) or (b) for consideration, or on any other commercial basis (“Commercial Purpose”), (iii) the buyer shall use Licensed Products and any Related Material in compliance with all applicable laws and regulations, including without limitation applicable human health and animal welfare laws and regulations, and (iv) the buyer shall indemnify, defend and hold harmless MIT, Harvard and The Broad and their current and former trustees, directors, officers, faculty, affiliated investigators, students, employees, and agents and their respective successors, heirs and assigns (“Indemnitees”), against any liability, damage, loss, or expense (including without limitation reasonable attorneys’ fees and expenses) incurred by or imposed upon any of the Indemnitees in connection with any claims, suits, investigations, actions, demands or judgments arising out of or related to the exercise of any rights granted to the buyer, or any breach of the rights granted hereunder by the buyer.

Guide-it Recombinant Cas9 (Electroporation-Ready) is a recombinant wild-type Streptococcus pyogenes Cas9 nuclease expressed with a C-terminal nuclear localization signal (NLS). The Cas9 protein solution has been verified to be sterile and well-tolerated by mammalian cells when electroporated as a ribonucleoprotein complex (RNP) with a single guide RNA (sgRNA) for knockout experiments, or as an RNP with a donor repair template for knockin experiments.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

Knock out of CD81 in induced pluripotent stem cells

Knock out of CD81 in induced pluripotent stem cells
Knock out of CD81 in induced pluripotent stem cells. In this experiment, two induced pluripotent stem cell lines, hiPSC-18 and hiPSC-22, were electroporated withCas9/sgRNA RNPs against CD81. Cells were stained for CD81 and then run on a FACS machine to detect CD81 ten days after editing. The negative staining control shows the FACS data from cells without antibody staining, while the electroporation control shows cells electroporated without Cas9/sgRNA RNPs. When cells were electroporated with both Cas9 and sgRNA, very high editing efficiency was observed.

.

Back

Homology-directed repair at the AAVS1 or CXCR4 genes

Homology-directed repair at the AAVS1 or CXCR4 genes

Homology-directed repair at the AAVS1 or CXCR4 genes. Panel A. These diagrams demonstrate how the HDR experiments in CD34+ HSCs were done. A ssdNA template containing a HindIII restriction site was inserted into the AAVS1 gene, and a template containing both HindIII and BamHI restriction sites was inserted into the CXCR4 gene. The homology arms were 90 bp on each side. Panel B. After electroporation of the repair template and Cas9/sgRNA RNPs, gene targets were amplified from target cells using PCR and analyzed by restriction digest. Editing efficiencies are shown above each positive well on the gel. The extra band in the BamHI digest of the CXCR4 gene is due to a second BamHI site already present in the wild-type gene before editing. We verified that 98% of the HSCs stained positive for CD34+ five days after editing.

.

Back

Gene knockouts in the Cellartis hiPSC-18 cell line

Gene knockouts in the Cellartis hiPSC-18 cell line

Gene knockouts in the Cellartis hiPSC-18 cell line. The strength of the cleaved bands gives a semiquantitative estimate of the percentage of edited cells. As can be seen, the Guide-it recombinant Cas9, when combined with sgRNA from the Guide-it In Vitro Transcription Kit, provides consistent and effective gene editing for many targets when compared with other vendors’ recommended methods for producing guide RNAs. Numbers at the bottom of each gel represent gene editing efficiencies (expressed as a %) for the indicated RNPs.

Back

632641: Guide-it Recombinant Cas9 (Electroporation-Ready)

632641: Guide-it Recombinant Cas9 (Electroporation-Ready)

*You must be logged in to a Business Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2019 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Support
  • Contact us
  • Technical support
  • Customer service
  • Shipping & delivery
  • Sales
  • Feedback
Products
  • New products
  • Special offers
  • Instrument & reagent services
  • Corporate development
Learning centers
  • NGS
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
About
  • Our brands
  • Careers
  • Events
  • Blog
  • Need help?
  • Announcements
  • Quality and compliance
  • That's Good Science!
Facebook Twitter  LinkedIn

©2019 Takara Bio Inc. All Rights Reserved.

Region - North America Privacy Policy Terms and Conditions Terms of Use

Top



  • Automation systems
  • SmartChip Real-Time PCR System, chips, and reagents
  • Apollo system
  • ICELL8 system and software
  • Next-generation sequencing
  • Single-cell RNA- and DNA-seq
  • RNA-seq
  • DNA-seq
  • Immune profiling
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Human iPS cell gene editing systems
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • cDNA synthesis
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion Cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Cell biology assays
  • Extracellular vesicle miniprep
  • Exosome isolation (cell culture)
  • Reporter systems
  • Apoptosis detection kits
  • Epigenetics
  • Signal transduction
  • Gene function
  • Gene editing
  • Fluorescent proteins
  • Viral transduction
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISAs
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • New products
  • Special offers
  • Fluorescent protein plasmids
  • Capturem his-tagged purification sale
  • In-Fusion Cloning sale
  • qPCR promotion
  • Nucleic acid purification sale
  • Nucleic acid purification kit samples
  • Power medium promotion
  • CRISPR-Cas9 promotion
  • Lenti-X special offers
  • PCR samples
Alzheimer's disease research

Alzheimer's disease research

Alzheimer's disease (AD) affects ~5.8 million adults and is the sixth leading cause of death in the US alone. Over 400 AD clinical trials are currently under way, investigating the possibilities of immunotherapy, our microbiome, and inflammation as prospective therapeutic targets. We have a variety of best-in-class products that can help your AD research move forward.

See how our best-in-class solutions can help to advance your AD research

  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • Apollo library prep system introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Selection guide
  • Product line overview
  • Technical notes
  • FAQs and tips
  • Webinars
  • Posters
  • Real-time PCR
  • Product finder
  • Reaction size guidelines for qPCR
  • Real-time PCR products brochure
  • Real-time PCR tutorial videos
  • Overview
  • Technical notes
  • FAQs
  • Stem cell research
  • Applications
  • Technical notes
  • Webinars
  • Videos
  • Protocols
  • Citations
  • Nucleic acid purification
  • Product finder
  • Plasmid purification
  • Genomic DNA purification
  • DNA/RNA cleanup and extraction
  • Automated DNA and RNA purification
  • RNA purification
  • Hard-to-lyse samples
  • cDNA synthesis
  • PCR
  • Citations
  • Selection guides
  • PCR enzyme brochure
  • Technical notes
  • PCR FAQs
  • Cloning
  • In-Fusion Cloning tools
  • In-Fusion Cloning guide
  • In‑Fusion Cloning FAQs
  • In‑Fusion Cloning tips
  • Choosing a seamless cloning method
  • Seamless cloning primer design
  • Cell biology assays
  • Extracellular vesicle isolation from biofluids
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Transfection reagents
  • Fluorescent proteins
  • Protein research
  • Capturem rapid purification technology
  • Antibody purification
  • His-tag purification
  • Phosphoprotein and glycoprotein purification
  • Mass spectrometry digestion reagents
  • Matchmaker Gold yeast two-hybrid systems
  • Antibodies and ELISA
Capturem Trypsin for a rapid, efficient mass spectometry workflow at room temperature.

Speed up your mass spec workflow

Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

Capturem trypsin technology

  • Customer service
  • Sales
  • Shipping & delivery
  • Technical support
  • Website FAQs
  • Feedback
  • Trademarks
  • License statements
  • Vector information
  • Vector document overview
  • Vector document finder
  • Online tools
  • Business development
  • OEM, custom, and supply-chain solutions
  • In licensing
  • Out licensing
  • Submit a licensing request
  • Instrument & reagent services
  • Cell and gene therapy manufacturing services
  • Custom enzyme supply services
  • Instrument services
  • Stem cell services
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
Takara Bio's award-winning GMP-compliant manufacturing facility in Kusatsu, Shiga, Japan.

Partner with Takara Bio!

Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan.

Learn more

  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
Create a web account with us

Log in to enjoy additional benefits

Want to save this information?

An account with takarabio.com entitles you to extra features such as:

•  Creating and saving shopping carts
•  Keeping a list of your products of interest
•  Saving all of your favorite pages on the site*
•  Accessing restricted content

*Save favorites by clicking the star () in the top right corner of each page while you're logged in.

Create an account to get started

  • Announcements
  • Events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Quality statement
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Our history
  • That's Good Science!
  • Season one
  • Season two
  • Season three
  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • About our blog
  • Need help?
Best-in-class products, expert support, superior value

That's GOOD Science!

What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

Explore what makes good science possible

 Customer Login
 View Cart (0)
  • Home
  • Products
  • Learning centers
  • Services & Support
  • Areas of interest
  • Feedback
  • About
  •  Customer Login
  • Register
  •  View Cart (0)

Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

  • Products
  • Automation systems
  • Next-generation sequencing
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Cell biology assays
  • Real-time PCR
  • cDNA synthesis
  • Automation systems
  • SmartChip Real-Time PCR System, chips, and reagents
  • Apollo system
  • ICELL8 system and software
  • Next-generation sequencing
  • Single-cell RNA- and DNA-seq
  • RNA-seq
  • DNA-seq
  • Immune profiling
  • Epigenetics and small RNA sequencing
  • NGS accessories
  • Gene function
  • Gene editing
  • Fluorescent proteins
  • Viral transduction
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • ProteoTuner protein control systems
  • iDimerize inducible protein interaction systems
  • Transfection reagents
  • Mammalian expression plasmids
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Human iPS cell gene editing systems
  • Accessories
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Expression vectors & systems
  • Glycobiology
  • Antibodies and immunoprecipitation
  • SDS-PAGE & western blotting
  • Protein sequencing
  • Accessory enzymes
  • PCR
  • Most popular polymerases
  • Standard PCR
  • High-yield PCR
  • High-fidelity PCR
  • Fast PCR
  • Long-range PCR
  • GC rich PCR
  • Direct PCR
  • PCR master mixes
  • Custom business friendly and automation-ready solutions
  • Molecular diagnostic products
  • GMP-grade products
  • Application-specific PCR
  • Other PCR-related products
  • PCR thermal cyclers
  • Cloning
  • In-Fusion Cloning
  • Competent cells
  • Ligation kits
  • Mutagenesis kits
  • Ligation enzymes
  • Restriction enzymes
  • Modifying enzymes
  • Legacy cloning products
  • X-Gal and IPTG
  • Linkers, primers, and cloning vectors
  • Agarose gel electrophoresis
  • Nucleic acid extraction
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • RNA cleanup kits
  • Viral DNA and RNA purification kits
  • Accessories and components
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Secondary antibodies
  • Antibody and ELISA accessories
  • Fluorescent protein antibodies
  • Cell biology assays
  • Extracellular vesicle miniprep
  • Exosome isolation (cell culture)
  • Reporter systems
  • Apoptosis detection kits
  • Epigenetics
  • Cell biology reagents
  • RNA interference
  • Cell-culture accessories
  • Signal transduction
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • Real-time PCR primer sets
  • References and standards for qPCR
  • RNA extraction and analysis for real-time qPCR
  • Application-specific qPCR
  • cDNA synthesis
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • cDNA synthesis accessories
  • Learning centers
  • Automation systems
  • Next-generation sequencing
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Cell biology assays
  • Real-time PCR
  • cDNA synthesis
  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • Apollo library prep system introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Selection guide
  • Product line overview
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Webinars
  • Citations
  • Posters
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Transfection reagents
  • Fluorescent proteins
  • Stem cell research
  • Applications
  • Technical notes
  • Posters
  • Webinars
  • Videos
  • Protocols
  • FAQs
  • Citations
  • Selection guides
  • Overview
  • Protein research
  • Capturem rapid purification technology
  • Antibody purification
  • His-tag purification
  • Other tag purification
  • Phosphoprotein and glycoprotein purification
  • Mass spectrometry digestion reagents
  • Matchmaker Gold yeast two-hybrid systems
  • Expression systems
  • PCR
  • Citations
  • Selection guides
  • PCR enzyme brochure
  • Technical notes
  • PCR FAQs
  • Go green with lyophilized enzymes
  • LA PCR technology
  • Cloning
  • In-Fusion Cloning tools
  • In-Fusion Cloning guide
  • In‑Fusion Cloning FAQs
  • In‑Fusion Cloning tips
  • Choosing a seamless cloning method
  • Seamless cloning primer design
  • In-Fusion Cloning applications collection
  • Efficient cloning for sgRNA/Cas9 plasmids
  • In-Fusion Cloning tech notes
  • In-Fusion Cloning webinars
  • In-Fusion Cloning citations
  • EcoDry reagents and sustainability
  • Mutagenesis with In-Fusion Cloning
  • Efficient multiple-fragment cloning
  • Sign up to stay updated
  • Traditional molecular cloning
  • Nucleic acid purification
  • Product finder
  • Plasmid purification
  • Genomic DNA purification
  • DNA/RNA cleanup and extraction
  • Parallel DNA, RNA & protein
  • Automated DNA and RNA purification
  • RNA purification
  • Hard-to-lyse samples
  • Antibodies and ELISA
  • Osteocalcin focus
  • Cell biology assays
  • Extracellular vesicle isolation from biofluids
  • Cell viability kits
  • Exosome isolation from cell culture
  • Mir-X microRNA quantification
  • Real-time PCR
  • Product finder
  • Reaction size guidelines for qPCR
  • Real-time PCR products brochure
  • Real-time PCR tutorial videos
  • Overview
  • Technical notes
  • FAQs
  • cDNA synthesis
  • Premium total and poly A+ RNA
  • SMARTer RACE 5'/3' Kit—advances in SMARTer PCR cDNA synthesis
  • Cloning antibody variable regions
  • Services & Support
  • Technical support
  • Shipping & delivery
  • Customer service
  • Sales
  • Website FAQs
  • Vector information
  • Online tools
  • Instrument & reagent services
  • Corporate development
  • Takara Bio affiliates & distributors
  • License statements
  • Trademarks
  • Vector information
  • Vector document overview
  • Vector document finder
  • Instrument & reagent services
  • Cell and gene therapy manufacturing services
  • Custom enzyme supply services
  • Instrument services
  • Stem cell services
  • Corporate development
  • OEM, custom, and supply-chain solutions
  • In licensing
  • Out licensing
  • Submit a licensing request
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Areas of interest
  • Cancer research
  • Immunotherapy research
  • Alzheimer's disease research
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • About
  • Manufacturing DSS Takara Bio India
  • Careers
  • Quality and compliance
  • Need help?
  • Our brands
  • Our history
  • Announcements
  • Our partners
  • BioView blog
  • That's Good Science!
  • Special offers
  • New products
  • Events
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • About our blog
  • That's Good Science!
  • Season one
  • Season two
  • Season three
  • Special offers
  • Buy Again and Save More on DSS Takara Bio India Manufactured products
  • Classic Campaign on Takara Bio and Macherey Nagel products
  • Fluorescent protein plasmids
  • Fluorescent protein plasmids
  • Capturem his-tagged purification sale
  • In-Fusion Cloning sale
  • qPCR promotion
  • Nucleic acid purification sale
  • Nucleic acid purification kit samples
  • Power medium promotion
  • CRISPR-Cas9 promotion
  • Lenti-X special offers
  • PCR samples
  • EOY Promo (DKK)
  • EOY Promo (EUR)
  • EOY Promo (SEK)
  • EOY Promo (CHF)
  • EOY Promo (GBP)
  • qPCR-RT promo (DKK)
  • RT-qPCR bundle promotion (EUR)
  • RT-qPCR bundle promotion (SEK)
  • RT-qPCR bundle promotion (CHF)
  • RT-qPCR bundle promotion (GBP)
  • Events
  • Calendar
  • Conferences
  • Speak with us
  • Products
  • Learning centers
  • Services & Support
  • Areas of interest
  • About