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Products >  Genome_Editing >  CRISPR_Cas9 >  Mutation_Detection_Kit

Guide-it Mutation Detection Kit

The Guide-it Mutation Detection Kit contains all the reagents needed for PCR-based identification of insertions or deletions generated in the genome of cells treated with engineered nucleases such as zinc-finger nucleases (ZFNs), transcription-activator-like effector nucleases (TALENs), or CRISPR/Cas9. The first step is the amplification of the putative target sequence directly from cells. This kit uses Terra PCR Direct Polymerase Mix and Buffer, so there is no need to extract genomic DNA from your cell population prior to amplification of your target sequence. The amplicon is then melted and hybridized to form the mismatched targets for cleavage by the Guide-it Resolvase. Agarose gel electrophoresis can be used to visualize the cleavage products.
  At-A-Glance   Documents   Images & Data   Resources

Features

  • Simple method to identify insertions or deletions in mammalian cells
  • Faster and more efficient than the surveyor assay; amplify directly from your target cells
  • Complete kit: Guide-it Resolvase, Terra PCR Direct Polymerase and all buffers included
  • Value: 100 reactions per kit; also available in a 25 reaction size for small-scale genome editing projects
  • Perfect for confirming sequence changes downstream from Cas9/CRISPR, TALENs or zinc-finger nuclease studies

Applications

  • Confirmation of mutations caused by genome editing technologies, including CRISPR/Cas9, TALENs, or zinc-finger nucleases
  • Detecting insertions and deletions in mammalian genomic DNA

Components

  • Extraction Buffer 1
  • Extraction Buffer 2
  • Guide-it Resolvase
  • Terra PCR Direct Polymerase Mix (1.25 U/µl)
  • 2X Terra PCR Direct Buffer (with Mg2+, dNTP)
  • PCR-Grade Water

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
631443 Guide-it™ Mutation Detection Kit 100 Rxns $428.00 License Statements
631448 Guide-it™ Mutation Detection Kit 25 Rxns $188.00 License Statements
 

 

 
 

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